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ISHI-Veg Protocols
Method validation is a critical aspect of method development in ISHI-Veg. Performance criteria, such as analytical specificity, analytical sensitivity, selectivity, repeatability and reproducibility are measured to demonstrate that the method is fit for purpose. The methods follow ISHI-Veg best practices for PCR, ELISA and dilution-plating assays used in routine seed health testing. The best practice documents identify the number and types of controls necessary for a robust seed health test.
ISHI-Veg methods are reviewed at regular intervals and revised, if found necessary. Therefore, it is the responsibility of the user to check that the most recent version of the protocol is being applied.
Contact the ISF Secretariat if you would like to know more about these methods.
Disclaimer
ISF cannot guarantee that labs following these protocols will obtain similar results. Many factors, such as staff skills, lab equipment and conditions, reagents and sampling methods can influence the results. Consequently, in case of any litigation ISF will not accept any liability on the use of these tests.
| Crop | Pest(s) | Method(s) | Publication Date | |
| Common Name | Scientific Name | |||
| Bean | Phaseolus vulgaris | Pseudomonas savastanoi pv. phaseolicola | Dilution plating Pathogenicity assay | July 2017 |
| Xanthomonas axonopodis pv. phaseoli Xanthomonas axonopodis pv. phaseoli var. fuscans | Dilution plating Identification qPCR Pathogenicity assay | October 2019 | ||
| Brassica | Brassica spp. | Phoma lingam | Blotter Plating | July 2017 |
| Xanthomonas campestris pv. campestris Xanthomonas campestris pv. raphani (untreated seed) | Seed extract qPCR Dilution plating Identification qPCR Pathogenicity assay | October 2019 | ||
| Xanthomonas campestris pv. campestris (disinfested/disinfected seed) | Dilution plating Confirmation PCR Pathogenicity assay | July 2017 | ||
| Carrot | Daucus carota | Alternaria dauci | Blotter Plating | July 2017 |
| Alternaria radicina | Blotter Plating (Malt Agar) | July 2017 | ||
| Plating (A. radicina selective agar) | July 2017 | |||
| Xanthomonas hortorum pv. carotae | Dilution plating Identification qPCR Pathogenicity assay | November 2019 | ||
| Celery, Celeriac | Apium graveolens | Septoria apiicola | Blotter Pathogenicity assay (seed wash) | February 2019 |
| Corn salad | Valerianella locusta | Acidovorax valerianellae | Sweat box grow-out Confirmation PCR | July 2017 |
| Cucurbit species | Cucurbitaceae | Acidovorax citrulli | Seed extract qPCR | May 2021 |
| Cucumber green mottle mosaic virus Melon necrotic spot virus Squash mosaic virus | ELISA | July 2017 | ||
| Lettuce | Lactuca sativa | Lettuce mosaic virus | ELISA (seed and seedlings) | July 2017 |
| Melon | Cucumis melo | Acidovorax citrulli | Sweat box grow-out Pathogenicity assay | May 2021 |
| Pea | Pisum sativum | Pea seed-borne mosaic virus Pea early browning virus | ELISA | July 2017 |
| Pseudomonas syringae pv. pisi | Dilution plating Identification PCR Pathogenicity assay | December 2020 | ||
| Pepper | Capsicum annuum | Tobamoviruses | ELISA Bioassay | September 2019 |
| Tomato brown rugose fruit virus | Seed extract qPCR Bioassay | November 2020 | ||
| Xanthomonas spp. | Dilution plating Identification PCR Pathogenicity assay | July 2017 | ||
| Spinach | Spinacia oleracea | Verticillium dahliae | Plating (un-treated seeds) Blotter (treated seeds) | July 2017 |
| Squash | Cucurbita pepo | Pseudomonas syringae | March 2022 | |
| Tomato | Solanum lycopersicum | Clavibacter michiganensis subsp. michiganensis | Dilution plating Identification PCR Pathogenicity assay | July 2017 |
| Pepino mosaic virus | ELISA Bioassay Confirmation ELISA | March 2021 | ||
| Tobamoviruses | ELISA Bioassay | July 2019 | ||
| Tomato brown rugose fruit virus | Seed extract qPCR Bioassay | November 2020 | ||
| Xanthomonas spp. | Dilution plating Identification PCR Pathogenicity assay | July 2017 | ||