ISHI-Veg methods are developed using the collective expertise and experience of its members.

ISHI-Veg Protocols

Method validation is a critical aspect of method development in ISHI-Veg. Performance criteria, such as analytical specificity, analytical sensitivity, selectivity, repeatability and reproducibility are measured to demonstrate that the method is fit for purpose. The methods follow ISHI-Veg best practices for PCR, ELISA and dilution-plating assays used in routine seed health testing. The best practice documents identify the number and types of controls necessary for a robust seed health test.

ISHI-Veg methods are reviewed at regular intervals and revised, if found necessary. Therefore, it is the responsibility of the user to check that the most recent version of the protocol is being applied.


Contact the ISF Secretariat if you would like to know more about these methods.

Disclaimer

ISF cannot guarantee that labs following these protocols will obtain similar results. Many factors, such as staff skills, lab equipment and conditions, reagents and sampling methods can influence the results. Consequently, in case of any litigation ISF will not accept any liability on the use of these tests.

CropPest(s)Method(s)Publication Date
Common NameScientific Name
BeanPhaseolus vulgarisPseudomonas savastanoi pv. phaseolicolaDilution plating
Pathogenicity assay
July 2017
Xanthomonas axonopodis pv. phaseoli
Xanthomonas axonopodis pv. phaseoli var. fuscans
Dilution plating
Identification qPCR
Pathogenicity assay
October 2019
BrassicaBrassica spp.Phoma lingamBlotter
Plating
July 2017
Xanthomonas campestris pv. campestris
Xanthomonas campestris pv. raphani
(untreated seed)
Seed extract qPCR
Dilution plating
Identification qPCR
Pathogenicity assay
October 2019
Xanthomonas campestris pv. campestris
(disinfested/disinfected seed)
Dilution plating
Confirmation PCR
Pathogenicity assay
July 2017
CarrotDaucus carotaAlternaria dauciBlotter
Plating
July 2017
Alternaria radicinaBlotter
Plating (Malt Agar)
July 2017
Plating (A. radicina selective agar)July 2017
Xanthomonas hortorum pv. carotaeDilution plating
Identification qPCR
Pathogenicity assay
November 2019
Celery, CeleriacApium graveolensSeptoria apiicolaBlotter
Pathogenicity assay (seed wash)
February 2019
Corn saladValerianella locustaAcidovorax valerianellaeSweat box grow-out
Confirmation PCR
July 2017
Cucurbit speciesCucurbitaceaeAcidovorax citrulliSeed extract qPCRMay 2021
Cucumber green mottle mosaic virus
Melon necrotic spot virus
Squash mosaic virus
ELISAJuly 2017
LettuceLactuca sativaLettuce mosaic virusELISA (seed and seedlings)July 2017
MelonCucumis meloAcidovorax citrulliSweat box grow-out
Pathogenicity assay
May 2021
PeaPisum sativumPea seed-borne mosaic virus
Pea early browning virus
ELISAJuly 2017
Pseudomonas syringae pv. pisiDilution plating
Identification PCR
Pathogenicity assay
December 2020
PepperCapsicum annuumTobamovirusesELISA
Bioassay
September 2019
Tomato brown rugose fruit virusSeed extract qPCR
Bioassay
November 2020
Xanthomonas spp.Dilution plating
Identification PCR
Pathogenicity assay
July 2017
SpinachSpinacia oleraceaVerticillium dahliaePlating (un-treated seeds)
Blotter (treated seeds)
July 2017
SquashCucurbita pepoPseudomonas syringae

Grow out

Plating

Pathogenicity assay

March 2022
TomatoSolanum lycopersicumClavibacter michiganensis subsp. michiganensisDilution plating
Identification PCR
Pathogenicity assay
July 2017
Pepino mosaic virusELISA
Bioassay
Confirmation ELISA
March 2021
TobamovirusesELISA
Bioassay
July 2019
Tomato brown rugose fruit virusSeed extract qPCR
Bioassay
November 2020
Xanthomonas spp.Dilution plating
Identification PCR
Pathogenicity assay
July 2017