Validation is a requirement of method development, a process that determines the fitness of a method for its intended purpose.

ISHI-Veg Validation Reports

ISHI-Veg’s detection methods are used for many purposes, such as preventing pathogens moving from one country to another, as a pre-screen to clear lots that are healthy for commercialization and for seed producers to make management decisions. To ensure its methods are fit for purpose, ISHI-Veg has identified 6 validation criteria against which the performance of the methods are measured, see ISHI-Veg Guidelines for the Validation of Seed Health Methods. ISHI-Veg has benchmarked its validation criteria against international validation standards and will continue to do so on a regular basis.

CropPest(s)Assay(s)Publication date
Common nameScientific name
BeanPhaseolus vulgaris

Xanthomonas axonopodis pv. phaseoli

Xanthomonas axonopodis pv. phaseoli var. fuscans

Identification qPCRFebruary 2019
CarrotDaucus carotaCandidatus Liberibacter solanacearumSeed extract qPCRJanuary 2020
Xanthomonas hortorum pv. carotaeIdentification qPCRJuly 2019

Dilution plating

Identification qPCR

August 2021
MelonCucumis meloAcidovorax citrulliSweat box grow-outApril 2021
PeaPisum sativumPseudomonas syringae pv. pisi Identification PCROctober 2020
PepperCapsicum annuumTomato brown rugose fruit virusSeed extract qPCRMarch 2020
SquashCucurbita pepoPseudomonas syringae



Pathogenicity assay

March 2022
TomatoSolanum lycopersicumTomato brown rugose fruit virusSeed extract qPCRMarch 2020

Contact the ISF Secretariat if you would like to know more about these reports.