Validation is a requirement of method development, a process that determines the fitness of a method for its intended purpose.
ISHI Validation Reports
The ISHI has a clear process for the development of its methods and the improvement of existing ones. The different phases of the process include project initiation, method development, method validation: in-house assessment, method validation including inter-laboratory comparative test, and project finalization. Validation is a critical aspect of method development and is intrinsically embedded into each project in ISHI. To ensure its methods are fit for purpose, ISHI has identified six validation criteria, which are analytical specificity, analytical sensitivity, selectivity, repeatability, reproducibility and diagnostic performance, against which the performance of the method is measured, see ISHI-Guidelines for the Validation of Seed Health Methods. The ISHI has benchmarked its validation criteria against international validation standards and will continue to do so on a regular basis.
The entire process of method development and method validation is conducted by a project team with a project lead. The scope of the method and its validation criteria are first defined and documented in a validation plan before the onset of the experimental studies. All results of the experiments and their analyses are then compiled in a validation report. Internal critical peer reviewing occurs at various stages of the process, by ISHI members independent of the project team, to ensure the development of fit-for-purpose methods that can be used widely and secure the delivery of healthy seed to customers.
The table below presents all validation reports.
Contact the ISF Secretariat if you would like to know more about these reports.
|Common name||Scientific name|
Xanthomonas axonopodis pv. phaseoli
Xanthomonas axonopodis pv. phaseoli var. fuscans
|Identification qPCR||February 2019|
|Carrot||Daucus carota||Candidatus Liberibacter solanacearum||Seed extract qPCR||January 2020|
|Xanthomonas hortorum pv. carotae||Identification qPCR||July 2019|
|Melon||Cucumis melo||Acidovorax citrulli||Sweat box grow-out||April 2021|
|Pea||Pisum sativum||Pseudomonas syringae pv. pisi||Identification PCR||October 2020|
|Pepper||Capsicum annuum||Tomato brown rugose fruit virus||Seed extract qPCR||March 2020|
|Squash||Cucurbita pepo||Pseudomonas syringae||March 2022|
|Tomato||Solanum lycopersicum||Clavibacter michiganensis subsp. michiganensis||Seed extract qPCR||July 2022|
|Pepino mosaic virus||Seed extract qPCR||June 2023|
|Tomato brown rugose fruit virus||Seed extract qPCR||March 2020|