Validation is a requirement of method development, a process that determines the fitness of a method for its intended purpose.

ISHI Validation Reports

The ISHI has a clear process for the development of its methods and the improvement of existing ones. The different phases of the process include project initiation, method development, method validation: in-house assessment, method validation including inter-laboratory comparative test, and project finalization. Validation is a critical aspect of method development and is intrinsically embedded into each project in ISHI. To ensure its methods are fit for purpose, ISHI has identified six validation criteria, which are analytical specificity, analytical sensitivity, selectivity, repeatability, reproducibility and diagnostic performance, against which the performance of the method is measured, see ISHI Technical ResourcesISHI-Guidelines for the Validation of Seed Health Methods. The ISHI has benchmarked its validation criteria against international validation standards and will continue to do so on a regular basis.

The entire process of method development and method validation is conducted by a project team with a project lead. The scope of the method and its validation criteria are first defined and documented in a validation plan before the onset of the experimental studies. All results of the experiments and their analyses are then compiled in a validation report. Internal critical peer reviewing occurs at various stages of the process, by ISHI members independent of the project team, to ensure the development of fit-for-purpose methods that can be used widely and secure the delivery of healthy seed to customers.

The table below presents all validation reports.

Contact the ISF Secretariat if you would like to know more about these reports.

CropPest(s)Assay(s)Publication date
Common nameScientific name
BeanPhaseolus vulgaris

Xanthomonas axonopodis pv. phaseoli

Xanthomonas axonopodis pv. phaseoli var. fuscans

Identification qPCRFebruary 2019
CarrotDaucus carotaCandidatus Liberibacter solanacearumSeed extract qPCRJanuary 2020
Xanthomonas hortorum pv. carotaeIdentification qPCRJuly 2019

Dilution plating

Identification qPCR

August 2021
MelonCucumis meloAcidovorax citrulliSweat box grow-outApril 2021
PeaPisum sativumPseudomonas syringae pv. pisi Identification PCROctober 2020
PepperCapsicum annuumTomato brown rugose fruit virusSeed extract qPCRMarch 2020
SquashCucurbita pepoPseudomonas syringae

Grow-out

Plating

Pathogenicity assay

March 2022
TomatoSolanum lycopersicumTomato brown rugose fruit virusSeed extract qPCRMarch 2020