ISHI methods are developed using the collective expertise and experience of its members.

ISHI Methods

The ISHI seed health tests are used for many purposes, such as preventing pathogens moving from one country to another, to clear lots that are healthy for commercialization and for seed producers to make management decisions. The methods developed follow ISHI best practices, and are reviewed at regular intervals and revised, if found necessary. Therefore, it is the responsibility of the user to check that the most recent version of the method is being applied.

The table below presents all the methods.

Contact the ISF Secretariat if you would like to know more about these methods.

Disclaimer

ISF cannot guarantee that laboratories following these methods will obtain similar results. Many factors, such as staff skills, laboratory equipment and conditions, reagents and sampling methods can influence the results. Consequently, in case of any litigation ISF will not accept any liability on the use of these methods.

Crop Pest(s) Method(s) Publication Date
Common Name Scientific Name
Bean Phaseolus vulgaris Pseudomonas savastanoi pv. phaseolicola Dilution plating Pathogenicity assay July 2017
Xanthomonas axonopodis pv. phaseoli Xanthomonas axonopodis pv. phaseoli var. fuscans Dilution plating Identification qPCR Pathogenicity assay October 2019
Brassica Brassica spp. Phoma lingam Blotter Plating July 2017
Xanthomonas campestris pv. campestris Xanthomonas campestris pv. raphani (untreated seed) Seed extract qPCR Dilution plating Identification qPCR Pathogenicity assay October 2019
Xanthomonas campestris pv. campestris (disinfested/disinfected seed) Dilution plating Confirmation PCR Pathogenicity assay July 2017
Carrot Daucus carota Alternaria dauci Blotter Plating July 2017
Alternaria radicina Blotter Plating (Malt Agar) July 2017
Plating (A. radicina selective agar) July 2017
Candidatus Liberibacter solanacearum Seed extract qPCR June 2020
Xanthomonas hortorum pv. carotae Dilution plating Identification qPCR Pathogenicity assay December 2021
Celery, Celeriac Apium graveolens Septoria apiicola Blotter Pathogenicity assay (seed wash) February 2019
Corn salad Valerianella locusta Acidovorax valerianellae Sweat box grow-out Confirmation PCR July 2017
Cucurbit species Cucurbitaceae Acidovorax citrulli Seed extract qPCR May 2021
Cucumber green mottle mosaic virus Melon necrotic spot virus Squash mosaic virus ELISA July 2017
Lettuce Lactuca sativa Lettuce mosaic virus ELISA (seed and seedlings) July 2017
Melon Cucumis melo Acidovorax citrulli Sweat box grow-out Pathogenicity assay May 2021
Pea Pisum sativum Pea seed-borne mosaic virus Pea early browning virus ELISA July 2017
Pseudomonas syringae pv. pisi Dilution plating Identification PCR Pathogenicity assay December 2020
Pepper Capsicum annuum Tobamoviruses ELISA Bioassay September 2019
Tomato brown rugose fruit virus Seed extract qPCR Bioassay November 2020
Xanthomonas spp. Dilution plating Identification PCR Pathogenicity assay July 2017
Spinach Spinacia oleracea Verticillium dahliae Plating (un-treated seeds) Blotter (treated seeds) July 2017
Squash Cucurbita pepo Pseudomonas syringae Grow out Plating Pathogenicity assay March 2022
Tomato Solanum lycopersicum Clavibacter michiganensis subsp. michiganensis Dilution plating Identification PCR Pathogenicity assay July 2017
Pepino mosaic virus ELISA Bioassay Confirmation ELISA March 2021
Pospiviroids Seed extract qPCR February 2018
Tobamoviruses ELISA Bioassay July 2019
Tomato brown rugose fruit virus Seed extract qPCR Bioassay November 2020
Xanthomonas spp. Dilution plating Identification PCR Pathogenicity assay July 2017