ISHI methods are developed using the collective expertise and experience of its members.

ISHI Methods

The ISHI seed health tests are used for many purposes, such as preventing pathogens moving from one country to another, to clear lots that are healthy for commercialization and for seed producers to make management decisions. The methods developed follow ISHI best practices, and are reviewed at regular intervals and revised, if found necessary. Therefore, it is the responsibility of the user to check that the most recent version of the method is being applied.

The table below presents all the methods.

Contact the ISF Secretariat if you would like to know more about these methods.

Disclaimer

ISF cannot guarantee that laboratories following these methods will obtain similar results. Many factors, such as staff skills, laboratory equipment and conditions, reagents and sampling methods can influence the results. Consequently, in case of any litigation ISF will not accept any liability on the use of these methods.

CropPest(s)Method(s)Publication Date
Common NameScientific Name
BeanPhaseolus vulgarisPseudomonas savastanoi pv. phaseolicola

Dilution plating

Pathogenicity assay

July 2017
Xanthomonas axonopodis pv. phaseoli
Xanthomonas axonopodis pv. phaseoli var. fuscans

Dilution plating

Identification qPCR

Pathogenicity assay

October 2019
BrassicaBrassica spp.Phoma lingam

Blotter

Plating

July 2017
Xanthomonas campestris pv. campestris
Xanthomonas campestris pv. raphani
(untreated seed)

Seed extract qPCR

Dilution plating

Identification qPCR
Pathogenicity assay

October 2019
Xanthomonas campestris pv. campestris
(disinfested/disinfected seed)

Dilution plating

Confirmation PCR

Pathogenicity assay

July 2017
CarrotDaucus carotaAlternaria dauci

Blotter

Plating

July 2017
Alternaria radicina

Blotter

Plating (Malt Agar)

July 2017
Plating (A. radicina selective agar)July 2017
Candidatus Liberibacter solanacearumSeed extract qPCRJune 2020
Xanthomonas hortorum pv. carotae

Dilution plating

Identification qPCR

Pathogenicity assay

December 2021
Celery, CeleriacApium graveolensSeptoria apiicolaBlotter
Pathogenicity assay (seed wash)
February 2019
Corn saladValerianella locustaAcidovorax valerianellae

Sweat box grow-out

Confirmation PCR

July 2017
Cucurbit speciesCucurbitaceaeAcidovorax citrulliSeed extract qPCRMay 2021
Cucumber green mottle mosaic virus
Melon necrotic spot virus
Squash mosaic virus
ELISAJuly 2017
LettuceLactuca sativaLettuce mosaic virusELISA (seed and seedlings)July 2017
MelonCucumis meloAcidovorax citrulli

Sweat box grow-out

Pathogenicity assay

May 2021
PeaPisum sativumPea seed-borne mosaic virus
Pea early browning virus
ELISAJuly 2017
Pseudomonas syringae pv. pisi

Dilution plating

Identification PCR

Pathogenicity assay

December 2020
PepperCapsicum annuumTobamoviruses

ELISA

Bioassay

September 2019
Tomato brown rugose fruit virus

Seed extract qPCR

Bioassay

November 2020
Xanthomonas spp.

Dilution plating

Identification PCR

Pathogenicity assay

July 2017
SpinachSpinacia oleraceaVerticillium dahliae

Plating (un-treated seeds)

Blotter (treated seeds)

July 2017
SquashCucurbita pepoPseudomonas syringae

Grow out

Plating

Pathogenicity assay

March 2022
TomatoSolanum lycopersicumClavibacter michiganensis subsp. michiganensis

SE-qPCR

Dilution plating

Identification PCR

Pathogenicity assay

July 2022

Pepino mosaic virus

ELISA

Bioassay

Confirmation ELISA

March 2021
PospiviroidsSeed extract qPCRFebruary 2018
Tobamoviruses

ELISA

Bioassay

July 2019
Tomato brown rugose fruit virus

Seed extract qPCR

Bioassay

November 2020
Xanthomonas spp.

Dilution plating

Identification PCR

Pathogenicity assay

July 2017