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ISHI Methods
The ISHI seed health tests are used for many purposes, such as preventing pathogens moving from one country to another, to clear lots that are healthy for commercialization and for seed producers to make management decisions. The methods developed follow ISHI best practices, and are reviewed at regular intervals and revised, if found necessary. Therefore, it is the responsibility of the user to check that the most recent version of the method is being applied.
The table below presents all the methods.
Contact the ISF Secretariat if you would like to know more about these methods.
Disclaimer
ISF cannot guarantee that laboratories following these methods will obtain similar results. Many factors, such as staff skills, laboratory equipment and conditions, reagents and sampling methods can influence the results. Consequently, in case of any litigation ISF will not accept any liability on the use of these methods.
| Crop | Pest(s) | Method(s) | Publication Date | |
| Common Name | Scientific Name | |||
| Bean | Phaseolus vulgaris | Pseudomonas savastanoi pv. phaseolicola | July 2017 | |
| Xanthomonas axonopodis pv. phaseoli Xanthomonas axonopodis pv. phaseoli var. fuscans | October 2019 | |||
| Brassica | Brassica spp. | Phoma lingam | July 2017 | |
| Xanthomonas campestris pv. campestris Xanthomonas campestris pv. raphani (untreated seed) | October 2019 | |||
| Xanthomonas campestris pv. campestris (disinfested/disinfected seed) | July 2017 | |||
| Carrot | Daucus carota | Alternaria dauci | July 2017 | |
| Alternaria radicina | July 2017 | |||
| Plating (A. radicina selective agar) | July 2017 | |||
| Candidatus Liberibacter solanacearum | Seed extract qPCR | December 2023 | ||
| Xanthomonas hortorum pv. carotae | December 2021 | |||
| Celery, Celeriac | Apium graveolens | Septoria apiicola | Blotter Pathogenicity assay (seed wash) | February 2019 |
| Corn salad | Valerianella locusta | Acidovorax valerianellae | July 2017 | |
| Peronospora valerianellae | Grow-out | April 2025 | ||
| Stagonosporopsis valerianellae | October 2024 | |||
| Cucurbit species | Cucurbitaceae | Acidovorax citrulli | Seed extract qPCR | May 2021 |
| Cucumber green mottle mosaic virus Melon necrotic spot virus Squash mosaic virus | ELISA | June 2025 | ||
| Lettuce | Lactuca sativa | Lettuce mosaic virus | ELISA (seed and seedlings) | July 2017 |
| Melon | Cucumis melo | Acidovorax citrulli | May 2021 | |
| Onion | Allium cepa | Botrytis allii | Plating | November 2023 |
| Pea | Pisum sativum | Pea seed-borne mosaic virus Pea early browning virus | ELISA | July 2017 |
| Pseudomonas syringae pv. pisi | December 2020 | |||
| Pepper | Capsicum annuum | Pospiviroids | Seed extract qPCR | March 2023 |
| Tobamoviruses | September 2019 | |||
| Tomato brown rugose fruit virus | Seed extract qPCR | April 2024 | ||
| Xanthomonas spp. | July 2017 | |||
| Spinach | Spinacia oleracea | Verticillium dahliae | July 2017 | |
| Squash | Cucurbita pepo | Pseudomonas syringae | March 2022 | |
| Tomato | Solanum lycopersicum | Clavibacter michiganensis subsp. michiganensis | July 2022 | |
| Pepino mosaic virus | June 2023 | |||
| Pospiviroids | Seed extract qPCR | March 2023 | ||
| Tobamoviruses | July 2019 | |||
| Tomato brown rugose fruit virus | Seed extract qPCR | April 2024 | ||
| Xanthomonas spp. | July 2017 | |||