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ISHI provides a forum for exchanging information, sharing expertise on seed health testing and the development of new seed health tests.

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ISHI shares its knowledge on seed health testing through various documents besides its methods, validation reports, and best practices.

Below are lists of guidelines, position papers, terms, technical fact sheets, publications and videos.

Guidelines

TitlePublication Year
Guidelines on Seed Health Testing in the Vegetable Seed Industry2010
Real-time PCR, an ‘indirect’ test used for pre-screening in seed health methods2018

Position Papers

TitlePublication Year
ISF Viewpoint on Indirect Seed Health Tests 2013

Technical Opinion Papers

TitlePublication Year
High Throughput Sequencing in Seed Health Testing2019
Tobamovirus outbreaks in Solanaceae are not linked to use of indicator plant tests: A retrospective analysis2022

Technical Guidelines

TitlePublication Year
Technical Guidelines for PCR Assay Development for Seed Health Tests2024
Technical Guidelines for the Organisation of Comparative Tests2024
Technical Guidelines for the Validation of Seed Health Methods2025

Terms

TitlePublication Year
Abbreviations2025
Glossary of Terms2025

Technical Fact Sheets

TitlePublication Year
Southern tomato virus in Tomato seed2019

Publications (from 2019)

TitleJournalPublication Year
ISHI in Retrospect: Let’s tackle this togetherProphyta – Focus on Europe2019
Tomato brown rugose fruit virus: ISHI-Veg develops new, specific detection methodProphyta – The Annual2019
The future of seed health testing: going back to basicsProphyta – The Annual2020
Global seed movement poses demanding challengesProphyta – The Annual2021
Detection Acidovorax citrulli in melon: faster, cheaper and more efficientProphyta – Focus on Europe2021
Zucchini vein clearing disease: ISHI-Veg develops grow-out based detection methodProphyta – The Annual2022
Seed Health Testing: Doing Things RightPhytoFrontiers™2022
Seed health initiative on vegetables: Celebrating 30 years of ISHI-VegProphyta – The Annual2023
Seed health methods: ISHI’s validation processProphyta – The Annual2023
Development and validation of real-time PCR tests for specific identification of Xanthomonas species causing bacterial spot disease on tomato (Solanum lycopersicum) and pepper (Capsicum annuum)EPPO Bulletin2023
International Seed Health Initiative: Improving toolbox to support evolving needsProphyta – The Annual2024
Development and validation of a multiplex real-time RT-PCR test for the screening of tomato (Solanum lycopersicum) for the detection of pepino mosaic virusEPPO Bulletin2024
Harmonization of controls: Building blocks of the ISHI best practicesProphyta – Focus on Europe2025
Updated ISHI method: New atypical strains of Clavibacter michiganensisProphyta – The Annual2026

Videos

TitlePublication Year
“Improved Assay” by ISHI-Veg (Pseudomonas syringae pv. pisi)2021
“New Assay” by ISHI-Veg (Tomato brown rugose fruit virus)2021
“Practical Alternative” by ISHI-Veg (Acidovorax citrulli)2021